Serum Metabolomic Profiling in Rheumatoid Arthritis Patients With Interstitial Lung Disease: A Case–Control Study

Frontiers in Medicine

article

Hiroshi Furukawa¹  Toshihiro Matsui³  Naoshi Fukui²  Kiyoshi Migita⁴  Shigeto Tohma¹  Shomi Oka¹  Kota Shimada³  Akira Okamoto⁷  Atsushi Hashimoto³  Akiko Komiya²  Koichiro Saisho^1,10  Norie Yoshikawa^1,10  Masao Katayama^1,12 

[1] Department of Rheumatology, National Hospital Organization Tokyo National Hospital;Clinical Research Center for Allergy and Rheumatology, National Hospital Organization Sagamihara National Hospital;Department of Rheumatology, National Hospital Organization Sagamihara National Hospital;Clinical Research Center, National Hospital Organization Nagasaki Medical Center;Department of Gastroenterology and Rheumatology, Fukushima Medical University School of Medicine;Department of Rheumatic Diseases, Tokyo Metropolitan Tama Medical Center;Department of Rheumatology, National Hospital Organization Himeji Medical Center;Department of Internal Medicine, Sagami Seikyou Hospital;Department of Clinical Laboratory, National Hospital Organization Sagamihara National Hospital;Department of Orthopedics/Rheumatology, National Hospital Organization Miyakonojo Medical Center;Tanimura Hospital;Department of Internal Medicine, National Hospital Organization Nagoya Medical Center

关键词: rheumatoid arthritis;    interstitial lung disease;    metabolomics;    usual interstitial pneumonia;    non-specific interstitial pneumonia;   

DOI  :  10.3389/fmed.2020.599794

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Objectives: Interstitial lung disease (ILD) is an extra-articular manifestation in rheumatoid arthritis (RA), detected in 10.7% of patients, and causing a poor prognosis. Hence, biomarkers for ILD are urgently required in RA. Low molecular weight metabolites can be assessed by metabolomic analyses, and although these have been conducted in RA and in idiopathic pulmonary fibrosis, few have been carried out for ILD in the context of RA. Therefore, we analyzed serum metabolomic profiles of ILD in RA to identify novel biomarkers. Methods: Serum samples from 100 RA patients with ILD and 100 matched RA patients without chronic lung disease (CLD) were collected. These samples were subjected to metabolomic analyses using capillary electrophoresis time-of-flight mass spectrometry. Results: A total of 299 metabolites were detected in the metabolomic analysis. By univariate analysis, serum levels of decanoic acid and morpholine were lower in RA with ILD (false discovery rate Q = 1.87 × 10 −11 and 7.09 × 10 −6 , respectively), and glycerol was higher ( Q = 1.20 × 10 −6 ), relative to RA without CLD. Serum levels of these metabolites in RA with usual interstitial pneumonia or RA with non-specific interstitial pneumonia were also altered. The partial least squares-discriminant analysis model generated from these three metabolites could successfully discriminate ILD in RA (area under the curve: 0.919, 95% confidence interval: 0.867–0.968, sensitivity 0.880, specificity 0.780). Conclusions: Serum levels of some metabolites were significantly different in RA with ILD compared with RA without CLD. It is concluded that metabolomic profiling will be useful for discovering candidate screening biomarkers for ILD in RA.

【 授权许可】

CC BY