Characteristics of Peripheral Lymphocyte Subsets in Patients With Acute-On-Chronic Liver Failure Associated With Hepatitis B

Frontiers in Medicine

article

Juan Li¹  Ying-Ren Zhao¹  Ying-Li He²  Chun-Hua Hu¹  Yi Chen²  Mi-Mi Zhou²  Zhi-Jie Gao¹  Meng-Jun Fu¹  Jing Wang²  Jian-Zhou Li¹  Tian-Yan Chen¹ 

[1] Department of Infectious Diseases, School of Medicine, First Affiliated Teaching Hospital, Xi'an Jiaotong University;School of Medicine, Institution of Hepatology, First Affiliated Teaching Hospital, Xi'an Jiaotong University;Shaanxi Clinical Research Center of Infectious Diseases

关键词: lymphocyte subsets;    hepatitis B virus;    acute-on-chronic liver failure;    immune response;    flow cytometry;   

DOI  :  10.3389/fmed.2021.689865

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Background and Aims: Acute-on-chronic liver failure (ACLF) is a rare, but dramatic clinical syndrome. There is substantial evidence suggesting that immunity-mediated inflammation plays an important role in HBV-ACLF. Our aim was to characterize the proportion and cell counts of peripheral blood lymphocyte subsets in acute-on-chronic liver failure patients caused by HBV infection. Methods: One hundred and seventeen patients were enrolled in this study, including those with HBV-related ACLF (HBV-ACLF; n = 70), and HBV related non-ACLF patients (HBV non-ACLF; n = 47). Demographics, clinical and laboratory data at hospital admission were retrospectively analyzed. The percentage and cell count of peripheral lymphocyte subsets were evaluated by flow cytometry. Comparison analysis was performed by t -test or non-parametric Mann–Whitney U -test. Actuarial probabilities of death were calculated by the Kaplan-Meier method. Results: Both circulating lymphocyte count and lymphocyte percentage were significantly reduced in patients with HBV-ACLF ( P < 0.001). The CD8 + T cell, CD4 + T cell, and CD16 + CD56 + NK cell counts were significantly decreased in HBV-ACLF. Consistently, flow cytometric analysis showed that CD8 + T cell counts were significantly decreased in non-survivors, while no significant differences were found in CD4 + T cell, CD19 + B cell, or CD56 + CD16 + NK cell counts. Furthermore, the group with the lower CD8 + T cell count displayed a significantly higher mortality rate compared with the group with the higher CD8 + T cell count. Conclusions: The abnormal prevalence of lymphocyte subsets may be important in the pathogenesis of HBV-ACLF. The decrease in CD8 + T cell counts may be related to poor survival in HBV-ACLF patients.

【 授权许可】

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