Ecological strategies of biological and chemical control agents on wildfire disease of tobacco ( Nicotiana tabacum L.)

BMC Microbiology

article

Liu, Tianbo¹  Gu, Yabing³  Zhou, Zhicheng²  Liu, Zhenghua³  Yin, Huaqun³  Qin, Chong³  Yi, Tuyong¹  Tao, Jiemeng⁴ 

[1] College of Plant Protection, Hunan Agricultural University;Central South Agricultural Experiment Station of China Tobacco;School of Minerals Processing and Bioengineering, Central South University;China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC

关键词: Biological control agent;    Endophytic bacterial community;    Community assembly;    Ecological process;   

DOI  :  10.1186/s12866-021-02237-8

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

To investigate the ecological effects of chemical and biological control methods on tobacco wildfire disease, a plot field experiment was conducted to compare the control efficiency and mechanisms of a chemical pesticide (kasugamycin wettable powder, KWP) and a biological control agent (BCA) through high-throughput sequencing of bacterial 16S rRNA genes. The results showed that the BCA displayed better performance in decreasing the disease index and morbidity of tobacco than the chemical pesticide. By monitoring the endophytic community within tobacco leaves, it was found that the control effects of these two methods might be mediated by different changes in the endophytic bacterial communities and community assembly patterns. The application of either method decreased the taxonomic diversity of the leaf endophytic community. Compared to the BCA, KWP showed a more significant effect on the endophytic community structure, while the endophytic community treated with the BCA was able to return to the original state, which presented much lower disease infection. The disease control efficiency of KWP and BCA treatments might be achieved by increasing the abundance of Sphingomonas and Streptophyta, respectively. Furthermore, an analysis of the ecological processes in community assembly indicated that the BCA strengthened the homogeneous and variable selection, while KWP enhanced ecological drift. The results suggested different control mechanisms between KWP and BCA treatments, which will help in developing diverse ecological strategies for plant disease control.

【 授权许可】

CC BY|CC0