Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm

BMC Microbiology

article

Yamasaki, Ryota¹  Kawano, Aki¹  Yoshioka, Yoshie¹  Ariyoshi, Wataru¹ 

[1] Division of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental University

关键词: Biofilm inhibition;    Oral bacteria;    Rhamnolipids;    Surfactin;   

DOI  :  10.1186/s12866-020-02034-9

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Bacteria survive in various environments by forming biofilms. Bacterial biofilms often cause significant problems to medical instruments and industrial processes. Techniques to inhibit biofilm formation are essential and have wide applications. In this study, we evaluated the ability of two types of biosurfactants (rhamnolipids and surfactin) to inhibit growth and biofilm formation ability of oral pathogenic bacteria such as Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus sanguinis. Rhamnolipids inhibited the growth and biofilm formation ability of all examined oral bacteria. Surfactin showed effective inhibition against S. sanguinis ATCC10556, but lower effects toward A. actinomycetemcomitans Y4 and S. mutans UA159. To corroborate these results, biofilms were observed by scanning electron microscopy (SEM) and confocal microscopy. The observations were largely in concordance with the biofilm assay results. We also attempted to determine the step in the biofilm formation process that was inhibited by biosurfactants. The results clearly demonstrated that rhamnolipids inhibit biofilm formation after the initiation process, however, they do not affect attachment or maturation. Rhamnolipids inhibit oral bacterial growth and biofilm formation by A. actinomycetemcomitans Y4, and may serve as novel oral drug against localized invasive periodontitis.

【 授权许可】

CC BY|CC0