Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Proteome Science

Hong Xiang¹  Huiyi Song¹  Jianjun Liu¹  Dong Shang²  Ni Lou³ 

[1] Clinical Laboratory of Integrative Medicine, First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, 116023, Dalian, P. R. China;Clinical Laboratory of Integrative Medicine, First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, 116023, Dalian, P. R. China;The Third Department of General Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, P. R. China;Institute (College) of Integrative Medicine, Dalian Medical University, Dalian, China;

关键词: Biofilm inhibition;    Label-free quantitative proteomics;    Lactobacillus rhamnosus;    Escherichia coli;    Coculture;   

DOI  :  10.1186/s12953-021-00172-0

来源: Springer

【 摘 要 】

BackgroundEscherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules.MethodsTo explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively.ResultsThe proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction.ConclusionsThese findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

【 授权许可】

CC BY