Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay

BMC Microbiology

article

Singh, Kamal¹  Kumari, Richa¹  Tripathi, Rajneesh¹  Gupta, Smita¹  Anupurba, Shampa¹ 

[1] Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University

关键词: NTM;    Multiplex PCR;    MTBC;    MOTT;    Mycobacterium avium complex;    Mycobacterium abscessus and Mycobacterium kansasii;   

DOI  :  10.1186/s12866-020-01952-y

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinically most common NTM to the species level from pulmonary samples. Out of 50 isolates, 26 were identified as Mycobacterium kansasii (MK), 20 were identified as Mycobacterium abscessus (MA) and 4 were identified as Mycobacterium avium complex (MAC) through multiplex PCR and further confirmed by sequencing. Our study showed that multiplex PCR assay is a simple, convenient, and reliable technique for detection and differential identification of major NTM species.

【 授权许可】

CC BY|CC0