Identification of fungi in Tunisian olive orchards: characterization and biological control potential

BMC Microbiology

article

Gharsallah, Houda¹  Ksentini, Ines¹  Naayma, Sourour¹  Hadj Taieb, Karama¹  Abdelhedi, Nour¹  Schuster, Christina³  Triki, Mohamed Ali¹  Ksantini, Mohieddine¹  Leclerque, Andreas³ 

[1] Laboratory of Improvement and Protection of Genetic Resources of Olive Tree, Tunisian Olive Institute, University of Sfax;University of Sousse, Higher Institute of Agronomic Sciences of Chott Meriem;Institute for Microbiology and Biochemistry, Geisenheim University;Department of Biology, Technische Universität Darmstadt

关键词: Biological control;    Fungi;    Insect pests;    Antagonism;    Phytopathogenicity;    Entomopathogenicity;   

DOI  :  10.1186/s12866-020-01997-z

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Olive production is the main agricultural activity in Tunisia. The diversity of fungi was explored in two different olive groves located in two distant geographical zones in Sfax (Tunisia) with different management practices. Fungal isolation was made from soil and the major olive tree pests, namely the Olive fly, Bactrocera oleae Gmelin (Diptera: Tephritidae), and the Olive psyllid, Euphyllura olivina Costa (Homoptera: Psyllidae). A total of 34 fungal isolates were identified according to their phenotypic, genotypic, biochemical and biological activities. Twenty fungal species were identified belonging to six different genera (Alternaria, Aspergillus, Cladosporium, Fusarium, Lecanicillium and Penicillium) by the analysis of their ITS1–5.8S–ITS2 ribosomal DNA region. Different bioassays performed in this work revealed that 25/34 (73.5%) of the identified fungal isolates showed an entomopathogenic and/or antagonistic activity, 9/34 (26.5%) of them displayed phytopathogenic features. Fungal species that showed entomopathogenic and/or antagonistic potentialities and that are non-phytopathogenic, (17/34; 50%) of our fungal isolates, could be explored for olive protection against fungal diseases and pests, and might have a future application as biocontrol agents.

【 授权许可】

CC BY|CC0