Identification of fungi in Tunisian olive orchards: characterization and biological control potential

BMC Microbiology

Christina Schuster¹  Andreas Leclerque¹  Nour Abdelhedi²  Ines Ksentini²  Mohieddine Ksantini²  Houda Gharsallah²  Mohamed Ali Triki²  Sourour Naayma²  Karama Hadj Taieb³ 

[1] Institute for Microbiology and Biochemistry, Geisenheim University, Geisenheim, Germany;Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany;Laboratory of Improvement and Protection of Genetic Resources of Olive Tree, Tunisian Olive Institute, University of Sfax, Airport Road, P.B. 1087, 3000, Sfax, Tunisia;Laboratory of Improvement and Protection of Genetic Resources of Olive Tree, Tunisian Olive Institute, University of Sfax, Airport Road, P.B. 1087, 3000, Sfax, Tunisia;University of Sousse, Higher Institute of Agronomic Sciences of Chott Meriem, 4042, Sousse, Tunisia;

关键词: Biological control;    Fungi;    Insect pests;    Antagonism;    Phytopathogenicity;    Entomopathogenicity;   

DOI  :  10.1186/s12866-020-01997-z

来源: Springer

【 摘 要 】

BackgroundOlive production is the main agricultural activity in Tunisia. The diversity of fungi was explored in two different olive groves located in two distant geographical zones in Sfax (Tunisia) with different management practices.ResultsFungal isolation was made from soil and the major olive tree pests, namely the Olive fly, Bactrocera oleae Gmelin (Diptera: Tephritidae), and the Olive psyllid, Euphyllura olivina Costa (Homoptera: Psyllidae). A total of 34 fungal isolates were identified according to their phenotypic, genotypic, biochemical and biological activities. Twenty fungal species were identified belonging to six different genera (Alternaria, Aspergillus, Cladosporium, Fusarium, Lecanicillium and Penicillium) by the analysis of their ITS1–5.8S–ITS2 ribosomal DNA region. Different bioassays performed in this work revealed that 25/34 (73.5%) of the identified fungal isolates showed an entomopathogenic and/or antagonistic activity, 9/34 (26.5%) of them displayed phytopathogenic features.ConclusionsFungal species that showed entomopathogenic and/or antagonistic potentialities and that are non-phytopathogenic, (17/34; 50%) of our fungal isolates, could be explored for olive protection against fungal diseases and pests, and might have a future application as biocontrol agents.

【 授权许可】

CC BY