Microbial and metabolomic profiles in correlation with depression and anxiety co-morbidities in diarrhoea-predominant IBS patients

BMC Microbiology

article

Liu, Tong¹  Gu, Xiang¹  Li, Li-Xiang¹  Li, Ming¹  Li, Bing¹  Cui, Xiao¹  Zuo, Xiu-li¹ 

[1] Department of Gastroenterology, Qilu Hospital, Cheloo College of Medicine, Shandong University;Laboratory of Translational Gastroenterology, Qilu Hospital, Cheloo College of Medicine, Shandong University;Robot engineering laboratory for precise diagnosis and therapy of GI tumour, Qilu Hospital, Cheloo College of Medicine, Shandong University

关键词: Irritable bowel syndrome;    Depression;    Anxiety;    Microbial community;    Metabolomics;   

DOI  :  10.1186/s12866-020-01841-4

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Psychological co-morbidities in irritable bowel syndrome (IBS) have been widely recognized, whereas less is known regarding the role of gut microbial and host metabolic changes in clinical and psychological symptoms in IBS. A total of 70 diarrhoea-predominant IBS (IBS-D) patients and 46 healthy controls were enrolled in this study. Stool and urine samples were collected from both groups for 16S rRNA gene sequencing and metabolomic analysis. The results showed that fecal microbiota in IBS-D featured depleted Faecalibacterium (adjusted P = 0.034), Eubacterium rectale group (adjusted P = 0.048), Subdoligranulum (adjusted P = 0.041) and increased Prevotella (adjusted P = 0.041). O-ureido-L-serine, 3,4-dihydroxybenzenesulfonic acid and (R)-2-Hydroxyglutarate demonstrated lower urinary concentrations in IBS-D patients. We further built correlation matrices between gut microbe abundance, differentiated metabolite quantities and clinical parameters. Dialister manifested negative association with IBS severity (r = − 0.285, P = 0.017), anxiety (r = − 0.347, P = 0.003) and depression level (r = − 0.308, P = 0.010). Roseburia was negatively associated with IBS severity (r = − 0.298, P = 0.012). Twenty metabolites correlated with anxiety or depression levels, including 3,4-dihydroxymandelaldehyde with SAS (r = − 0.383, P = 0.001), 1-methylxanthine with SDS (r = − 0.347, P = 0.004) and 1D-chiro-inositol with SAS (r = − 0.336, P = 0.005). In analysis of microbe-metabolite relationship, 3,4-dihydroxymandelaldehyde and 1-methylxanthine were negatively correlated with relative abundance of Clostridiumsensu stricto. Our findings demonstrated altered microbial and metabolomic profiles associated with clinically and psychological symptoms in IBS-D patients, which may provide insights for further investigations.

【 授权许可】

CC BY|CC0