Morin inhibits Listeria monocytogenes virulence in vivo and in vitro by targeting listeriolysin O and inflammation

BMC Microbiology

article

Li, Gen¹  Wang, Guizhen³  Li, Meng¹  Li, Li²  Liu, Hongtao²  Sun, Meiyang⁴  Wen, Zhongmei¹ 

[1] Department of Respiratory Medicine, The First Hospital of Jilin University, Jilin University;Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University;College of Food Engineering, Jilin Engineering Normal University;Department of Breast Surgery, Jilin Provincial Cancer Hospital

关键词: Listeria monocytogenes;    Listeriolysin O;    morin;    Inflammation;    Anti-infection;   

DOI  :  10.1186/s12866-020-01807-6

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Listeria monocytogenes (L. monocytogenes) is a global opportunistic intracellular pathogen that can cause many infections, including meningitis and abortion in humans and animals; thus, L. monocytogenes poses a great threat to public safety and the development of the aquaculture industry. The isolation rate of Listeria monocytogenes in fishery products has always been high. And the pore-forming toxin listeriolysin O (LLO) is one of the most important virulence factors of L. monocytogenes. LLO can promote cytosolic bacterial proliferation and help the pathogen evade attacks from the host immune system. In addition, L. monocytogenes infection can trigger a series of severe inflammatory reactions. Here, we further confirmed that morin lacking anti-Listeria activity could inhibit LLO oligomerization. We also found that morin can effectively alleviate the inflammation induced by Listeria in vivo and in vitro and exerted an obvious protective effect on infected cells and mice. Morin does not possess anti-Listeria activity, neither does it interfere with secretion of LLO. However, morin inhibits oligomerisation of LLO and morin does reduce the inflammation caused during Listeria infection.

【 授权许可】

CC BY|CC0