The Oxidoreductase DsbA1 negatively influences 2,4-diacetylphloroglucinol biosynthesis by interfering the function of Gcd in Pseudomonas fluorescens 2P24

BMC Microbiology

article

Zhang, Bo¹  Zhao, Hui²  Wu, Xiaogang¹  Zhang, Li-Qun² 

[1] College of Agriculture, Guangxi University;College of Plant Protection, China Agricultural University

关键词: Pseudomonas fluorescens;    2;    4-DAPG;    Disulfide bond;    Oxidoreductase DsbA1;    Glucose dehydrogenase Gcd;   

DOI  :  10.1186/s12866-020-1714-1

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

The polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG), produced by Pseudomonas fluorescens 2P24, is positively regulated by the GacS-GacA two-component system. Here we reported on the characterization of DsbA1 (disulfide oxidoreductase) as novel regulator of biocontrol activity in P. fluorescens. Our data showed that mutation of dsbA1 caused the accumulation of 2,4-DAPG in a GacA-independent manner. Further analysis indicated that DsbA1 interacts with membrane-bound glucose dehydrogenase Gcd, which positively regulates the production of 2,4-DAPG. Mutation of cysteine (C)-235, C275, and C578 of Gcd, significantly reduced the interaction with DsbA1, enhanced the activity of Gcd and increased 2,4-DAPG production. Our results suggest that DsbA1 regulates the 2,4-DAPG concentration via fine-tuning the function of Gcd in P. fluorescens 2P24.

【 授权许可】

CC BY|CC0