The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) -mediated resistance

Journal of plant interactions

article

Vincent P. Klink¹  Omar Darwish²  Nadim W. Alkharouf³  Katherine S. Lawrence⁴ 

[1] a USDA-ARS-NEA-BARC Molecular Plant Pathology Laboratory;b Department of Mathematics and Computer Science, Texas Women’s University;c Department of Computer and Information Sciences, Towson University;d Department of Entomology and Plant Pathology, Auburn University

关键词: Plant;    transformation;    plasmid;    pRAP15;    pRAP17 overexpression;    heterologous expression;    ectopic expression;    RNA interference (RNAi);    crop;    genetic;    engineering;    nematode;    BRI1-ASSOCIATED RECEPTOR KINASE 1;    BAK1;   

DOI  :  10.1080/17429145.2021.1940328

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta (cassava) , Zea mays (maize) , Oryza sativa (rice) , Triticum aestivum (wheat) , Sorghum bicolor (sorghum) , Brassica rapa (rape seed) , Solanum tuberosum (potato) , Solanum lycopersicum (tomato) , Elaes guineensis (oil palm) , Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs .

【 授权许可】

CC BY|CC BY-NC