Investigation on salt-response mechanisms in Arabidopsis thaliana from UniProt protein knowledgebase

Journal of plant interactions

article

Meili Guo¹  Xin Liu²  Jiahui Wang²  Lei Li³  Wendi Zhang²  Benjiao Gong²  Chenglin Zhang²  Chunxi Zhou⁴ 

[1] a Department of Food and Biochemistry Engineering, Yantai Vocational College;b Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University;c Medical Experiment and Test Center, Capital Medical University;d Bruker Daltonics Inc., Beijing Office

关键词: Arabidopsis thaliana;    saltresponse;    cross-tolerance;    multiple locations;    bioinformatic prediction;    experimental validation;    UniProt protein knowledgebase;   

DOI  :  10.1080/17429145.2018.1551581

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Salt stress negatively affects plant growth and crop productivity. As an ideal model pathway of salt tolerance in glycophyte. To better understand the molecular mechanisms of salt-response in glycophyte, 466 of 15,768 Arabidopsis thaliana proteins with the GO term of biological with known genetic background, Arabidopsis thaliana has been widely applied to disclose the process ‘response to salt stress’ were retrieved from UniPort and analyzed by bioinformatics tools of PANTHER, DAVID, KEGG, Cytoscape and STRING. Our results not only indicated the involvement of salt-responsive proteins in various pathways and interaction networks, but also demonstrated the more complicated cross-tolerances to both abiotic stresses (osmosis, water deprivation, abscisic acid, cold, heat, light and wounding) and biotic stresses (bacterium and fungus) and multiple subcellular locations of these salt-responsive proteins. Furthermore, protein activities of superoxide dismutase (SOD) and peroxidase (POD) in Arabidopsis thaliana were determined under salt, cold and osmotic stresses, which validated the hypothesis of cross-tolerance to multiple stresses. Our work will greatly improve the current knowledge of salt tolerance mechanism in glycophytes and provide potential salt-responsive candidates for promoting plant growth and increasing crop output.

【 授权许可】

CC BY|CC BY-NC