期刊论文详细信息
Journal of plant interactions
Spatial expression of the Arabidopsis hydroperoxide lyase gene is controlled differently from that of the allene oxide synthase gene
article
Cynthia Mugo Mwenda1  Atsushi Matsuki2  Kohji Nishimura3  Takao Koeduka2  Kenji Matsui1 
[1] Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University;Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University;Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Organization of Research, Shimane University
关键词: oxylipin pathway;    hydroperoxide lyase;    allene oxide synthase;    green leaf volatiles;    jasmonates;    mechanical wounding;   
DOI  :  10.1080/17429145.2014.999836
学科分类:纳米科学和纳米技术
来源: Taylor & Francis
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【 摘 要 】

The hydroperoxide lyase (HPL) pathway for six carbon (C6) volatiles and the allene oxide synthase (AOS) pathway for jasmonates (JAs) share the first part of the pathway. To avoid competition, a separate localization of HPL and AOS might be important. A fusion protein comprising Arabidopsis HPL and green fluorescent protein was transported into chloroplasts, where AOS was located. Arabidopsis harboring β-glucuronidase (GUS) gene downstream of Arabidopsis HPL promoter (pAtHPL::GUS) showed different GUS activity in floral organs compared with that from pAtAOS::GUS. With pAtHPL::GUS, wounding enhanced GUS activity at the periphery of cotyledons; while with pAtAOS::GUS, GUS activity was high in the vasculature. The distribution of the ability to form C6 volatiles correlated with the profile of HPL promoter activity; however, this ability unchanged after wounding. Inconsistency between the AOS promoter activity and JA levels was also evident. Thus, an additional factor should also control the ability to form C6 volatiles and JAs.

【 授权许可】

CC BY|CC BY-NC   

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