Oxylipin-specific cytochrome P450s (CYP74s) in Lotus japonicus : their implications in response to mechanical wounding and nodule formation

Journal of plant interactions

article

Katsuyuki Yanagi¹  Koichi Sugimoto¹  Kenji Matsui¹ 

[1] Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University;Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University

关键词: oxylipin;    Lotus japonicus;    allene oxide synthase;    hydroperoxide lyase;    symbiosis;    jasmonate;   

DOI  :  10.1080/17429145.2011.562985

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Plant oxylipins are involved in defense responses against pathogens or herbivores. Each oxylipin compound exerts its distinctive physiological function. The ability of many legumes to fix nitrogen with rhizobia gives them special importance in natural environments and agriculture. It has been postulated that oxylipins are somehow related to symbiosis; however, this is still a controversial issue. In this study, we isolated five genes at the branching point of the oxylipin pathway in Lotus japonicus , and their biochemical functions were identified as allene oxide synthases (AOSs), 13-hydroperoxide lyase (13HPL), and 9/13-HPLs. When the leaves were mechanically wounded, AOS and 9/13HPL were upregulated in leaves and roots, respectively, from which their implications in wound response were suggested. When the plants were inoculated with rhizobia, no big change in the expression levels of genes was found. When high N was supplied to the nodulated plants, the number of nodules decreased, and simultaneously, AOS in the leaves was downregulated. Significance of AOS in response to the N status in the plants was suggested.

【 授权许可】

CC BY|CC BY-NC