Induction of β-1,3-glucanase and chitinase in Vigna aconitifolia inoculated with Macrophomina phaseolina

Journal of plant interactions

article

Shruti Shree Pareek¹  Indu Ravi²  Vinay Sharma¹ 

[1] Department of Bioscience and Biotechnology, Banasthali University;Indira Gandhi National Open University, IGNOU Regional Centre

关键词: pathogenesis-related proteins;    β-1;    3-glucanase;    chitinase;    Vigna aconitifolia;    Macrophomina phaseolina;   

DOI  :  10.1080/17429145.2013.849362

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Pathogenesis-related (PR) proteins are induced in response to pathogen attack. In the present study, the induction of PR proteins in response to the fungal pathogen Macrophomina phaseolina was investigated in 15-day- and 1-month-old plants of Vigna aconitifolia with resistant and susceptible cultivars. Inoculation of the fungal pathogen resulted in the enzyme activity gradually increased throughout the experimental period of 168 h compared to control. However, the activation of β-1,3-glucanase and chitinase was more rapid and to a greater extent in the resistant FMM-96 cultivar as compared to susceptible RM0-40 and CZM-3 cultivars. Furthermore, the western blot analysis revealed the presence of 33- and 30-kDa bands of β-1,3-glucanase and chitinase in induced moth bean plants, respectively. The possible implications of these findings as part of the general defense response of moth bean plants against the fungal pathogen (M. phaseolina) have been discussed.

【 授权许可】

CC BY|CC BY-NC