Changes in peripheral blood oxidative stress markers and hepatic gene expression related to oxidative stress in Holstein cows with and without subacute ruminal acidosis during the periparturient period

The Journal of Veterinary Medical Science

article

Yoshiyuki TSUCHIYA¹  Reiko OZAI³  Toshihisa SUGINO⁴  Kenji KAWASHIMA⁵  Shiro KUSHIBIKI⁶  Yo-Han KIM³  Shigeru SATO¹ 

[1] Graduate School of Veterinary Sciences, Iwate University;Yamagata Prefectural Agricultural Mutual Aid Association;Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University;The Research Center for Animal Science, Graduate School of Integrated Science for Life, Hiroshima University;Chiba Prefectural Livestock Research Center;Institute of Livestock and Grassland Science

关键词: hepatic gene expression;    Holstein cow;    oxidative stress;    periparturient period;    subacute ruminal acidosis;   

DOI  :  10.1292/jvms.20-0426

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

We investigated changes in peripheral blood metabolites, oxidative stress markers (malondialdehyde, potential antioxidant capacity, and glutathione peroxidase [GPX]), and hepatic gene expression related to oxidative stress in Holstein cows with and without subacute ruminal acidosis (SARA) during the periparturient period. Eighteen multiparous Holstein cows were categorized into SARA (n=9) or non-SARA (n=9) groups depending on whether they developed SARA; reticulo-ruminal pH was <5.6 for more than 3 hr per day, during the 2 weeks after parturition. Blood and liver tissue samples were collected 3 weeks prepartum and 2 and 6 weeks postpartum, with an additional blood sample collected 0 and 4 weeks postpartum. Blood aspartate transaminase (AST) and nonesterified fatty acid (NEFA) increased significantly ( P <0.05) after parturition in both groups. GPX activity decreased gradually after parturition in the SARA group. In the SARA group, gene expression of GPX 1 and microsomal glutathione S-transferase 3 ( MGST3 ) decreased significantly ( P <0.05), and expression of metallothionein 2A increased significantly ( P <0.05) after parturition in the SARA group. Superoxide dismutase 1 and MGST3 decreased significantly ( P <0.05) 2 weeks postpartum in the non-SARA group. Gene expression related to oxidative stress was negatively correlated with AST, NEFA and total ketone body levels. Therefore, the hepatic gene expression related to oxidative stress might change associated with a negative energy balance, and might relate the high oxidative stress in the SARA group during periparturient period.

【 授权许可】

Unknown