Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries

The journal of physiological sciences

article

Hyeong-Kyu Jeon¹  Keeseon S. Eom¹ 

[1] Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine

关键词: Spirometra erinaceieuropaei;    Spirometra decipiens;    mitochondria;    DNA sequence variability;   

DOI  :  10.3347/kjp.2019.57.5.481

来源: Springer

【 摘 要 】

Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.

【 授权许可】

Unknown