Pueraria lobata Root Constituents as Xanthine Oxidase Inhibitors and Protective Agents against Oxidative Stress Induced in GES-1 Cells

Journal of the Brazilian Chemical Society

article

Tang, Xiaosheng¹  Xiao, Aiping²  Mei, Shiyong²  Tang, Ping³  Ren, Licheng⁴  Liu, Liangliang² 

[1] Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, National Demonstration Center for Experimental Biology Education, College of Life Sciences, Hubei Normal University;Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences;Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation, School of Environmental Science and Engineering, Hubei Polytechnic University;Department of Plastic and Cosmetic Surgery, Shenzhen University General Hospital

关键词: fluorescence spectra;    inhibitor screening;    oxidative stress;    Pueraria lobata root;    xanthine oxidase;   

DOI  :  10.21577/0103-5053.20200108

学科分类：内科医学

来源: SciELO

【 摘 要 】

Ultrafiltration coupled with liquid chromatography-mass spectrometry (LC-MS) was established to screen xanthine oxidase (XO) inhibitors from Pueraria lobata root extract. Four compounds were screened out and identified as puerarin, daidzin, daidzein and genistein with half-maximal inhibitory concentration (IC 50 ) values of 30.8, 5.31, 14.5 and 3.02 µg mL −1 on XO, respectively. The interactions between these compounds and XO were investigated by fluorescence spectroscopic method. The hydrogen peroxide induced oxidative stress model of human normal gastric epithelial cell lines (GES-1) was used to investigate the protections on injured cell. As a result, four XO inhibitors exhibited protective effects without cytotoxicity. With the increased concentrations of four inhibitors, cell viability was higher with decreased mortality rate, the decrease of superoxide dismutase activity, leakage of lactate dehydrogenase and increase of intracellular superoxide anion production induced by hydrogen peroxide were restrained. It showed that these four XO inhibitors could effectively enhance cell viability and protect injury of GES-1 cells from oxidative stress.

【 授权许可】

CC BY