Molecular Characterisation of the GdhA - Derivative of Pasteurella multocida B:2

Pertanika Journal of Tropical Agricultural Science

article

Mohd. Zamri-Saad¹  Sarah Othman²  Teerasak E-kobon3³  Pramote Chumnanpuen⁴  Raha Abdul Rahim²  Farahani Muhammad Azam² 

[1] Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia;Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia;UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia;Department of Zoology, Faculty of Science, Kasetsart University;Department of Genetics, Faculty of Science, Kasetsart University

关键词: Haemorrhagic septicaemia;    live-attenuated vaccine;    OMPs;    Pasteurella multocida;    REP-PCR;   

DOI  :  10.47836/pjtas.44.1.10

学科分类：农业科学（综合）

来源: Universiti Putra Malaysia, Universiti Pertanian Malaysia Press

【 摘 要 】

Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate, P. multocida B:2 GDH7 was reported to enable protection in cattle and buffaloes via intranasal (i. n.) administration. This potential vaccine was also reported to be self-transmitted from the vaccinated animal to the free-ranging animals allowing wider vaccination coverage. Prior to commercialisation, this potential vaccine requires further characterisation in accordance with the authoritative guidelines from the World Organisation for Animal Health (OIE). Hence, in this study, the potential vaccine strain, P. multocida B:2 GDH7 and the virulent parent strain were characterised through genomic and proteomic profiling. A crucial first step was to develop a sensitive yet simple and robust identification test to differentiate both strains which has been achieved by the development of a precise yet straightforward PCR method. In genomic profiling, Repetitive Extragenic Palindromic sequence-PCR (REP-PCR) was manipulated and both strains have a different display of genomic DNA band patterns. Some of the major OMPs were observed and prominent immunogens of P. multocida, OmpA and OmpH were observed to be expressed differently between these strains through SDS-PAGE analysis. In conclusion, a reproducible PCR detection method has enabled differentiation of both strains. Further characterisation of these strains shows a significantly different profile through genomic and proteomic profiling.

【 授权许可】

Unknown