Immune response to dna vaccine expressing transferrin binding protein a gene of Pasteurella multocida

Brazilian Journal of Microbiology

Satparkash Singh²  Vijendra Pal Singh²  Pawanjit Singh Cheema²  Maninder Sandey¹  Rajeev Ranjan²  Santosh Kumar Gupta²  Bhaskar Sharma¹ 

[1] ,Indian Veterinary Research Institute Division of Bacteriology & Mycology ,India

关键词: DNA vaccine;    Haemorrhagic septicaemia;    IL2;    Pasteurella multocida;    Transferrin binding protein;   

DOI  :  10.1590/S1517-83822011000200043

来源: SciELO

【 摘 要 】

Haemorrhagic Septicaemia (HS), an acute and fatal disease of cattle and buffalo is primarily caused by serotype B:2 or E:2 of Pasteurella multocida. The transferrin binding protein A (TbpA) has been found to act as immunogen and potent vaccine candidate in various Gram negative bacteria including P. multocida. The present study was carried out to evaluate the potential of this antigen as a DNA vaccine against HS in mice model. The tbpA gene of P. multocida serotype B:2 was cloned in a mammalian expression vector alone and along with murine IL2 gene as immunological adjuvant to produce monocistronic and bicistronic DNA vaccine constructs, respectively. The immune response to DNA vaccines was evaluated based on serum antibody titres and lymphocyte proliferation assay. A significant increase in humoral and cell mediated immune responses was observed in mice vaccinated with DNA vaccines as compared to non immunized group. Additionally, the bicistronic DNA vaccine provided superior immune response and protection level following challenge as compared to monocistronic construct. The study revealed that DNA vaccine presents a promising approach for the prevention of HS.

【 授权许可】

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