Helicobacter -stimulated IL-10-producing B cells suppress differentiation of lipopolysaccharide/ Helicobacter felis -activated stimulatory dendritic cells

Turkish journal of biology

article

DOĞUŞ ALTUNÖZ¹  AYÇA SAYI YAZGAN¹ 

[1] Department of Molecular Biology and Genetics, Faculty of Science and Letters, İstanbul Technical University

关键词: Helicobacter felis;    regulatory B cells;    dendritic cells;    lipopolysaccharide;   

DOI  :  10.3906/biy-2012-11

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Regulatory B cells (Bregs) produce antiinflammatory cytokines and inhibits proinflammatory response. Recently, immunosuppressive roles of Bregs in the effector functions of dendritic cells (DCs) were demonstrated. However, cross talk between Bregs and DCs in Helicobacter infection remains unknown. Here, we showed that direct stimulation of bone marrow-derived DCs (BMDCs) with Helicobacter felis ( H. felis ) antigen upregulates their CD86 surface expression and causes the production of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). Furthermore, prestimulation of DCs with supernatants derived from both Helicobacter -stimulated IL-10 - B (Hf stim -IL-10 - B) or IL-10 + B (Hf stim -IL-10 + ) cells suppresses the secretion of TNF-α and IL-6, but does not affect the expression of CD86 and secretion of IL-12 by lipopolysaccharide (LPS) or H. felisactivated BM-DCs. Remarkably, soluble factors secreted by Hf stim -IL-10 - B cells, but not by Hf stim -IL-10 + B cells, suppress the secretion of IL-10 by BM-DCs upon subsequent LPS stimulation. In contrast, prestimulation with BM-DCs with supernatants of Hf stim -IL-10 + B cells before H. felis antigen stimulation induces significantly their IL-10 production. Collectively, our data indicated that prestimulation with soluble factors secreted by Hf stim -IL-10 + B cells, DCs exhibit a tolerogenic phenotype in response to LPS or Helicobacter antigen by secreting high levels of IL-10, but decreased levels of IL-6 and TNF-α.

【 授权许可】

Unknown