Comparison of anticarcinogenic properties of Viburnum opulus and its active compound p-coumaric acid on human colorectal carcinoma

Turkish journal of biology

article

SERDAR KARAKURT¹  GÜLSÜM ABUŞOĞLU²  ZEKİYE CEREN ARITULUK³ 

[1] Department of Biochemistry, Faculty of Science, Selçuk University;Department of Medical Laboratory Techniques, Vocational School of Health, Selçuk University;Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

关键词: Colorectal carcinoma;    Viburnum opulus;    p-coumaric acid;    TP53;    BRAF;    apoptosis;    cell cycle;   

DOI  :  10.3906/biy-2002-30

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Resistance to therapeutic agents and the highly toxic side effects of synthetic drugs has spurred new research in the treatment of colon cancer, which has high morbidity and mortality ratios. This study aims to clarify the molecular mechanisms of the anticarcinogenic properties of methanol extract of Viburnum opulus L. (EVO)and its main active compound, trans - p -coumaric acid ( p -CA), on human colon cancer cells (DLD-1, HT-29, SW-620, Caco-2) and healthy colon epithelial cells (CCD-18Co). The effects of EVO on controlled cell death (apoptosis) and the cell division cycle were determined by flow cytometry. Alteration in mRNA and protein expressions of switch genes in colorectal carcinoma (APC, MLH1, TP53, SMAD4, KRAS, and BRAF) were determined by qRT-PCR and Western blot, respectively. Our results show that EVO possesses a strong reducing capacity and free-radical scavenging activity. HPLC analyses prove that p -CAis the main compound of EVO. EVO and p -CA inhibit the proliferation of human colon cancer cells DLD-1 and HT-29 in a dose-dependent manner. EVO increases apoptosis of DLD-1 cells and halts the cell cycle in the G2 stage in HT-29 cells. mRNA and protein expressions of p53 and SMAD-4 are upregulated, while BRAFs are downregulated. The results were directly proportional to p-CA. EVO and p -CA up- and downregulate switch genes and protein expressions of DLD-1 cells, which alter the expression of 186 other genes. This is the first study of pharmacological exploration of I V.opulus in human colon cancer. Its antiproliferative effects may be due to the presence of p -CA.

【 授权许可】

Unknown