Changes to the identity of EndoC-βH1 beta cells may be mediated by stress-induced depletion of HNRNPD

Cell & Bioscience

Nicola Jeffery¹  Lorna W. Harries¹  David Chambers²  Ryan M. Ames³  Brandon M. Invergo⁴ 

[1] Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, Barrack Road, EX2 5DW, Exeter, UK;Kings College London, WC2R 2LS, London, UK;University of Exeter, Stocker Road, EX4 4QD, Exeter, UK;University of Exeter, Stocker Road, EX4 4QJ, Exeter, UK;

关键词: Beta-cells;    Cell differentiation;    HNRNPD;    RNA binding proteins;   

DOI  :  10.1186/s13578-021-00658-6

来源: Springer

【 摘 要 】

BackgroundBeta cell identity changes occur in the islets of donors with diabetes, but the molecular basis of this remains unclear. Protecting residual functional beta cells from cell identity changes may be beneficial for patients with diabetes.ResultsA somatostatin-positive cell population was induced in stressed clonal human EndoC-βH1 beta cells and was isolated using FACS. A transcriptomic characterisation of somatostatin-positive cells was then carried out. Gain of somatostatin-positivity was associated with marked dysregulation of the non-coding genome. Very few coding genes were differentially expressed. Potential candidate effector genes were assessed by targeted gene knockdown. Targeted knockdown of the HNRNPD gene induced the emergence of a somatostatin-positive cell population in clonal EndoC-βH1 beta cells comparable with that we have previously reported in stressed cells.ConclusionsWe report here a role for the HNRNPD gene in determination of beta cell identity in response to cellular stress. These findings widen our understanding of the role of RNA binding proteins and RNA biology in determining cell identity and may be important for protecting remaining beta cell reserve in diabetes.

【 授权许可】

CC BY