Genome-wide analysis and expression patterns of lipid phospholipid phospholipase gene family in Brassica napus L.

BMC Genomics

Xiling Zou¹  Yong Cheng¹  Xiaoyu Ding¹  Yan Lv¹  Ali Raza¹  Wei Su¹  Liu Zeng¹  Ang Gao¹ 

[1] Oil Crops Research Institute, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Chinese Academy of Agricultural Sciences (CAAS), Ministry of Agriculture, 430062, Wuhan, Hubei, China;

关键词: Abiotic stress;    Gene structure;    Gene ontology;    miRNA;    Phytohormone;    Lipid phosphate phosphatases;    Rapeseed;   

DOI  :  10.1186/s12864-021-07862-1

来源: Springer

【 摘 要 】

BackgroundLipid phosphate phosphatases (LPP) are critical for regulating the production and degradation of phosphatidic acid (PA), an essential signaling molecule under stress conditions. Thus far, the LPP family genes have not been reported in rapeseed (Brassica napus L.).ResultsIn this study, a genome-wide analysis was carried out to identify LPP family genes in rapeseed that respond to different stress conditions. Eleven BnLPPs genes were identified in the rapeseed genome. Based on phylogenetic and synteny analysis, BnLPPs were classified into four groups (Group I-Group IV). Gene structure and conserved motif analysis showed that similar intron/exon and motifs patterns occur in the same group. By evaluating cis-elements in the promoters, we recognized six hormone- and seven stress-responsive elements. Further, six putative miRNAs were identified targeting three BnLPP genes. Gene ontology analysis disclosed that BnLPP genes were closely associated with phosphatase/hydrolase activity, membrane parts, phosphorus metabolic process, and dephosphorylation. The qRT-PCR based expression profiles of BnLPP genes varied in different tissues/organs. Likewise, several gene expression were significantly up-regulated under NaCl, PEG, cold, ABA, GA, IAA, and KT treatments.ConclusionsThis is the first report to describe the comprehensive genome-wide analysis of the rapeseed LPP gene family. We identified different phytohormones and abiotic stress-associated genes that could help in enlightening the plant tolerance against phytohormones and abiotic stresses. The findings unlocked new gaps for the functional verification of the BnLPP gene family during stresses, leading to rapeseed improvement.

【 授权许可】

CC BY