Isolation and efficacy of two bacterial strains antagonists of Erwinia amylovora and Pectobacterium carotovorum

Egyptian Journal of Biological Pest Control

M’hamed BENADA¹  Boualem BOUMAAZA²  Omar KHALADI³  Sofiane BOUDALIA⁴ 

[1]Département des Sciences Agronomiques, Faculté des Sciences de la Nature et de la Vie, Université Ahmed Zabana Relizane, 48000, Relizane, Algérie

[2]Département des Sciences Agronomiques, Faculté des Sciences de la Nature et de la Vie, Université Ibn Khaldoun, 14000, Tiaret, Algérie

[3]Laboratoire de Biologie, Eau et Environnement, Université 8 Mai 1945 Guelma BP 4010, 24000, Guelma, Algérie

[4]Département d’Écologie et Génie de l’Environnement, Université 8 Mai 1945 Guelma BP 4010, 24000, Guelma, Algérie

[5]Laboratoire de Biologie, Eau et Environnement, Université 8 Mai 1945 Guelma BP 4010, 24000, Guelma, Algérie

[6]Département d’Écologie et Génie de l’Environnement, Université 8 Mai 1945 Guelma BP 4010, 24000, Guelma, Algérie

关键词: Biocontrol;    Erwinia amylovora;    Pseudomonas fluorescens;    Serratia plymuthica;    Antagonism;   

DOI  :  10.1186/s41938-021-00443-0

来源: Springer

【 摘 要 】

BackgroundThe development of ecofriendly tools against plant diseases is an important issue in crop protection. Screening and selection process of bacterial strains antagonists of 2 pathogenic bacterial species that limit very important crops, Erwinia amylovora, the causal agent of the fire blight disease, and Pectobacterium carotovorum, the causal agent of bacterial potato soft rot, were reported. Bacterial colonies were isolated from different ecological niches, where both pathogens were found: rhizosphere of potato tubers and fruits and leaves of pear trees from the northwest region of Algeria. Direct and indirect confrontation tests against strains of E. amylovora and P. carotovorum were performed.ResultsResults showed a significant antagonistic activity against both phytopathogenic species, using direct confrontation method and supernatants of cultures (p<0.005). In vitro assays showed growth inhibitions of both phytopathogenic species. Furthermore, results revealed that the strains of S. plymuthica had a better inhibitory effect than the strains of P. fluorescens against both pathogens. In vivo results on immature pear fruits showed a significant decrease in the progression of the fire blight symptoms, with a variation in the infection index from one antagonistic strain to another between 31.3 and 50%, and slice of potato showed total inhibition of the pathogen (P. carotovorum) by the antagonistic strains of Serratia plymuthica (p<0.005).ConclusionThis study highlighted that the effective bacteria did not show any infection signs towards plant tissue, and considered as a potential strategy to limit the fire blight and soft rot diseases.

【 授权许可】

CC BY