Bioresources and Bioprocessing | |
Simultaneous degradation of two mycotoxins enabled by a fusion enzyme in food-grade recombinant Kluyveromyces lactis | |
Zifeng Wu1  Yangyu Qiu1  Rui He1  Yahui Gao1  Qianqian Cheng1  Yu Xia2  Xiaoyuan Ma2  Zhouping Wang2  | |
[1] State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China;School of Food Science and Technology, Jiangnan University, 214122, Wuxi, China;State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China;School of Food Science and Technology, Jiangnan University, 214122, Wuxi, China;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 214122, Wuxi, China; | |
关键词: Mycotoxins; Aflatoxin B; Zearalenone; Fusion enzyme; Degradation; Kluyveromyces lactis; Food-grade; | |
DOI : 10.1186/s40643-021-00395-1 | |
来源: Springer | |
【 摘 要 】
Aflatoxin B1 (AFB1) and zearalenone (ZEN) are two predominant mycotoxins ubiquitously found in corn, peanuts, and other grains, which pose a great threat to human health. Therefore, safe and effective methods for detoxification of these mycotoxins are urgently needed. To achieve simultaneous degradation of multiple mycotoxins, a fusion enzyme ZPF1 was constructed by linking zearalenone hydrolase and manganese peroxidase with a linker peptide GGGGS. This fusion enzyme was secretory expressed successfully in the newly constructed food-grade recombinant strain Kluyveromyces lactis GG799(pKLAC1-ZPF1), and was investigated with the mycotoxins degradation efficiency in two reaction systems. Results showed that both AFB1 and ZEN can be degraded by ZPF1 in reaction system 1 (70.0 mmol/L malonic buffer with 1.0 mmol/L MnSO4, 0.1 mmol/L H2O2, 5.0 µg/mL AFB1 and ZEN, respectively) with the ratios of 46.46% and 38.76%, respectively. In reaction system 2 (50.0 mmol/L Tris–HCl, with 5.0 µg/mL AFB1 and ZEN, respectively), AFB1 cannot be degraded while ZEN can be degraded with the ratio of 35.38%. To improve the degradation efficiency of these mycotoxins, optimization of the induction and degradation conditions were fulfilled subsequently. The degradation ratios of AFB1 and ZEN by ZPF1 in reaction system 1 reached 64.11% ± 2.93% and 46.21% ± 3.17%, respectively. While in reaction system 2, ZEN was degraded by ZPF1 at a ratio of 41.45% ± 3.34%. The increases of degradation ratios for AFB1 and ZEN in reaction system 1 were 17.65% and 7.45%, respectively, while that for ZEN in reaction system 2 was 6.07%, compared with the unoptimized results.
【 授权许可】
CC BY
【 预 览 】
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RO202108122886544ZK.pdf | 2044KB | download |