Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize

BMC Plant Biology

Zhangying Xi¹  Guojun Li²  Mingshun Li²  Xinhai Li²  Degui Zhang²  Yong Chen²  Xiaonan Wang²  Ping Luo²  Yueheng Zhou²  Bojuan Liu²  Hongjun Yong²  Zhuanfang Hao²  Ming Cheng³  Nan Wang⁴  Xuecai Zhang⁵  Felix San Vicente⁵ 

[1] College of Agronomy, Henan Agricultural University, Zhengzhou, P.R. China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China;College of Agronomy, Henan Agricultural University, Zhengzhou, P.R. China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China;International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico;College of Agronomy, Hebei Agricultural University, Baoding, P.R. China;State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, P.R. China;International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico;

关键词: Zea Mays;    NAC transcription factor;    Natural variations;    Non-coding region;    Drought tolerance;   

DOI  :  10.1186/s12870-021-03072-9

来源: Springer

【 摘 要 】

BackgroundNatural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection.ResultsIn the present study, the NAC-encoding gene of ZmNAC080308 was cloned and sequenced in 199 inbred lines in maize. Phylogenetic analysis showed that ZmNAC080308 is closely clusteredinto the same group with other well-known NAC genes responding to improve drought tolerance. In total, 86 SNPs and 47 InDels were identified in the generic region of ZmNAC080308, 19 of these variations were associated with GY (grain yield) in different environments. Nine variations in the 5’-UTR region of ZmNAC080308 are closely linked, they might regulate the gene expression and respond to improve GY under drought condition via Sp1-mediated transactivation. Two haplotypes (Hap1 and Hap2) identified in the, 5’-UTR region using the nine variations, and Hap2 containing insertion variants, exhibited 15.47 % higher GY under drought stress condition. Further, a functional marker was developed to predict the drought stress tolerance in a US maize inbred line panel. Lines carrying Hap2 exhibited > 10 % higher GY than those carrying Hap1 under drought stress condition. In Arabidopsis, overexpression ZmNAC080308 enhanced drought tolerance.ConclusionsZmNAC080308 is an important gene responding to drought tolerance, a functional marker is developed for improving maize drought tolerance by selecting this gene.

【 授权许可】

CC BY