Physiological quality and amylase enzyme expression in maize seeds

Ciência e Agrotecnologia

Gustavo Evangelista Oliveira²  Renzo Garcia Von Pinho¹  Thaís De Andrade¹  Édila Vilela De Resende Von Pinho¹  Custódio Donizete Dos Santos¹  Adriano Delly Veiga¹ 

[1] ,Universidade Federal de Lavras Departamento de Biologia Lavras MG ,Brasil

关键词: Zea Mays;    seed quality;    qRT-PCR;    Zea Mays;    qualidade de sementes;    qRT-PCR;   

DOI  :  10.1590/S1413-70542013000100005

来源: SciELO

【 摘 要 】

The physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines.

【 授权许可】

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