Phytotoxicity, cytotoxicity and chemical composition of Spondias mombin Linn. Stem bark

Clinical Phytoscience

Patrick Ojeifo Uadia¹  Kissinger Obaogie Orumwensodia²  Muhammed Iqbal Choudhary³ 

[1] Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria;Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria;H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, 75270, Karachi, Pakistan;H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, 75270, Karachi, Pakistan;

关键词: Spondias mombin;    Phytotoxicity;    Cytotoxicity;    Lemna aequinocitalis;    Brine shrimp;    GC-MS;   

DOI  :  10.1186/s40816-021-00297-x

来源: Springer

【 摘 要 】

BackgroundSpondias mombin Linn. is a tropical climate plant with wide applications in ethnomedicinal practice. This study evaluates the phytotoxicity, cytotoxicity and chemical composition of the plant’s stem bark.MethodsDried stem bark sample of Spondias mombin Linn. was subjected to exhaustive extraction and partitioned into sub-fractions (hexane-ethylacetate, ethylacetate, ethylacetate-methanol and methanol) by graded polarity technique. The phytotoxicity and cytotoxicity indices of the crude hydro-ethanol extract and fractions were evaluated using Lemna minor and brine shrimp lethality assays, respectively, while chemical composition of the oily hexane:ethylacetate fraction was determined by gas chromatography-mass spectroscopy (GC-MS) technique.ResultsPhytotoxicity was dose-dependent which ranged from low (crude plant extract), moderate (hexane-ethylacetate and methanol fractions), high (ethylaacetate-methanol fraction) to significant toxicity (ethylacetate fraction) at the highest dose. However, for brine shrimp lethality assay only hexane-ethylacetate (LD50: 284.02 μg/mL) and ethylacetate (LD50: 210.24 μg/mL) fractions were cytotoxic at the highest dose. The GC-MS profile of the oily hexane:ethylacetate fraction identified sixty-eight compounds comprising hydrocarbons, fatty acids, alcohols, steroids, nitrogen and fluoride-containing compounds, terpenes and esters.ConclusionThis study concludes that fractions of Spondias mombin Lin. could be potentially toxic. While its phytotoxic potential can be useful in the agrochemical industry for the production of natural herbicides, its cytotoxic property can be cautiously harnessed for ethnomedicinal purposes.

【 授权许可】

CC BY