Genetic mapping and QTL analysis for peanut smut resistance

BMC Plant Biology

Marina Bressano¹  Carolina Ballén-Taborda²  Mario I. Buteler³  Juan H. Soave³  Sara J. Soave³  Melina Rosso³  Cecilia I. Bruno⁴  Claudio Oddino⁵  Beatriz P. Costero⁶  Francisco J. de Blas⁷  Eva Mamani⁸  J. Guillermo Seijo⁹  Alicia N. Massa^1,10  Renee S. Arias^1,10 

[1] Biología Celular, FCA - UNC, Av. Ing. Agr. Félix A. Marrone 735, CP5001, Córdoba, Argentina;Center for Applied Genetic Technologies and Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, USA;Criadero El Carmen, Bv. Italia 835, CP5809, Gral. Cabrera, Córdoba, Argentina;Estadística y Biometría, FCA – UNC, Córdoba, Argentina;CONICET, Av. Ing. Agr. Félix A. Marrone 735, CP5001, Córdoba, Argentina;Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto (FAV-UNRC), Ruta Nacional 36, X5804BYA, Córdoba, Argentina;Criadero El Carmen, Bv. Italia 835, CP5809, Gral. Cabrera, Córdoba, Argentina;Genética, Facultad de Ciencias Agropecuarias – UNC, Av. Ing. Agr. Félix A. Marrone 735, CP5001, Córdoba, Argentina;Instituto Multidisciplinario de Biología Vegetal Consejo Nacional de Investigaciones en Ciencia y Tecnología (CONICET) y Universidad Nacional de Córdoba (UNC), Av. Vélez Sarsfield 1666, X5016GCN, Córdoba, Argentina;Genética, Facultad de Ciencias Agropecuarias – UNC, Av. Ing. Agr. Félix A. Marrone 735, CP5001, Córdoba, Argentina;Instituto Nacional Tecnología Agropecuaria (INTA), Ruta Nac. nro. 9 km 636 Estación Experimental Agropecuaria Manfredi, EEA, X5988 Manfredi, Córdoba, Argentina;Instituto de Botánica del Nordeste (CONICET-UNNE) and Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Corrientes, Argentina;USDA-ARS-National Peanut Research Laboratory (NPRL), 39842, Dawson, GA, USA;

关键词: Arachis;    Disease resistance;    Introgression;    Peanut;    Groundnut;    QTL;    Peanut smut;    RIL population;   

DOI  :  10.1186/s12870-021-03023-4

来源: Springer

【 摘 要 】

BackgroundPeanut smut is a disease caused by the fungus Thecaphora frezii Carranza & Lindquist to which most commercial cultivars in South America are highly susceptible. It is responsible for severely decreased yield and no effective chemical treatment is available to date. However, smut resistance has been identified in wild Arachis species and further transferred to peanut elite cultivars. To identify the genome regions conferring smut resistance within a tetraploid genetic background, this study evaluated a RIL population {susceptible Arachis hypogaea subsp. hypogaea (JS17304-7-B) × resistant synthetic amphidiploid (JS1806) [A. correntina (K 11905) × A. cardenasii (KSSc 36015)] × A. batizocoi (K 9484)4×} segregating for the trait.ResultsA SNP based genetic map arranged into 21 linkage groups belonging to the 20 peanut chromosomes was constructed with 1819 markers, spanning a genetic distance of 2531.81 cM. Two consistent quantitative trait loci (QTLs) were identified qSmIA08 and qSmIA02/B02, located on chromosome A08 and A02/B02, respectively. The QTL qSmIA08 at 15.20 cM/5.03 Mbp explained 17.53% of the phenotypic variance, while qSmIA02/B02 at 4.0 cM/3.56 Mbp explained 9.06% of the phenotypic variance. The combined genotypic effects of both QTLs reduced smut incidence by 57% and were stable over the 3 years of evaluation. The genome regions containing the QTLs are rich in genes encoding proteins involved in plant defense, providing new insights into the genetic architecture of peanut smut resistance.ConclusionsA major QTL and a minor QTL identified in this study provide new insights into the genetic architecture of peanut smut resistance that may aid in breeding new varieties resistant to peanut smut.

【 授权许可】

CC BY