BMC Gastroenterology | |
Increased Vδ1γδT cells predominantly contributed to IL-17 production in the development of adult human post-infectious irritable bowel syndrome | |
B. L. Huang1  Z. C. Ma1  X. N. Sun1  Cheng Lan1  L. W. Dong1  J. Fu1  F. J. Liu1  D. C. Liang1  D. M. Sun2  | |
[1] Department of Gastroenterology, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical University, 570311, Haikou, Hainan Province, China;Doheny Eye Institute, Department of Ophthalmology, David Geffen School of Medicine, University of California, Los Angeles,, 90033, Los Angeles, CA, USA; | |
关键词: Post-infectious irritable bowel syndrome; Γδ T cells; Subset; Function; Pathogenesis; | |
DOI : 10.1186/s12876-021-01722-8 | |
来源: Springer | |
【 摘 要 】
BackgroundγδT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that γδ T cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study’s aim is to investigate the functionally predominant subset and its role in PI-IBS.MethodsThe total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of Vδ1 and Vδ2 subset was detected by FACS after stained with anti δ1-PE and anti δ2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. Vδ1 γδ T cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method and cultured, functionally evaluated by CCK-8 assay (proliferation), CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively.Results1. Vδ1 γδ T cells significantly increased while Vδ2 γδ T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (p < 0.05). 2. When cultured in vitro, the Vδ1 γδ T cells remarkably proliferated, activated and produced IL-17 (p < 0.05).ConclusionsOur results suggest that Vδ1 γδ T cells was the predominant γδ T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing γδ T cells in PI-IBS.
【 授权许可】
CC BY
【 预 览 】
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