qMrdd2, a novel quantitative resistance locus for maize rough dwarf disease

BMC Plant Biology

Zhaobang Cheng¹  Chunmei Ren¹  Qingcai Liu²  Mingliang Xu²  Suining Deng²  Weixiao Zhang³  Baoshen Liu³  Wei Xu³  Yongzhong Zhang³  Yan Zhao³ 

[1] Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, People’s Republic of China;National Maize Improvement Center, China Agricultural University, 2 West Yuanmingyuan Road, 100193, Beijing, People’s Republic of China;State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Street, 271018, Taian, People’s Republic of China;

关键词: Maize;    MRDD;    QTL;    Fine-mapping;    MAS;   

DOI  :  10.1186/s12870-021-03107-1

来源: Springer

【 摘 要 】

BackgroundMaize rough dwarf disease (MRDD), a widespread disease caused by four pathogenic viruses, severely reduces maize yield and grain quality. Resistance against MRDD is a complex trait that controlled by many quantitative trait loci (QTL) and easily influenced by environmental conditions. So far, many studies have reported numbers of resistant QTL, however, only one QTL have been cloned, so it is especially important to map and clone more genes that confer resistance to MRDD.ResultsIn the study, a major quantitative trait locus (QTL) qMrdd2, which confers resistance to MRDD, was identified and fine mapped. qMrdd2, located on chromosome 2, was consistently identified in a 15-Mb interval between the simple sequence repeat (SSR) markers D184 and D1600 by using a recombinant inbred line (RIL) population derived from a cross between resistant (“80007”) and susceptible (“80044”) inbred lines. Using a recombinant-derived progeny test strategy, qMrdd2 was delineated to an interval of 577 kb flanked by markers N31 and N42. We further demonstrated that qMrdd2 is an incompletely dominant resistance locus for MRDD that reduced the disease severity index by 20.4%.ConclusionsA major resistance QTL (qMrdd2) have been identified and successfully refined into 577 kb region. This locus will be valuable for improving maize variety resistance to MRDD via marker-assisted selection (MAS).

【 授权许可】

CC BY