Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene

Biological research: BR

article

Li, Yan¹  Zhou, Jinhua¹  Wang, Juan¹  Chen, Xiaoping²  Zhu, Yan²  Chen, Youguo¹ 

[1] Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University;Department of Obstetrics and Gynecology, The First People’s Hospital of Yancheng

关键词: miR-30b-3p;    Ovarian cancer;    OVCAR3;    CTHRC1;    EMT;   

DOI  :  10.1186/s40659-020-00277-4

来源: BioMed Central

【 摘 要 】

The aim of this study was to investigate the effect role and mechanism of miR-30b-3p on ovarian cancer cells biological function. The expression of miR-30b-3p was detected in ovarian cancer cell lines and normal ovarian epithelial cell line by qRT-PCR. Mir-30b-3p mimic was transfected into OVCAR3 cells. Cell-counting kit-8 (CCK-8) assay was conducted to explore the effect of mir-30b-3p on the OVCAR3 cells’ proliferation. Cell cycle and apoptosis were detected by Flow cytometry. Cell invasion ability was detected by Transwell test. The regulation of putative target of miR-30b-3p was verified by double luciferase reporter assays and Western blot. We found that miR-30b-3p was downregulated in OVCAR3 cells. Overexpression of miR-30b-3p suppressed proliferation, promoted apoptosis, slowed cell cycle and inhibited migration and invasion of OVCAR3 cells. Bioinformatics analysis identified 3′-untranslated region (3′UTR) of Collagen triple helix repeat-containing 1 (CTHRC1) as the presumed binding site for miR-30b-3p. Detection of double luciferase reporter and Western-Blot result confirmed that CTHRC1 was the target gene of miR-30b-3p. Furthermore, E-cadherin, β-cadherin and Vimentin protein expression level were changed after transfection of miR-30b-3p. miR-30b-3p function as an anti-cancer gene. Overexpression of miR-30b-3p can inhibit the biological function of ovarian cancer cells. MiR-30b-3p targets CTHRC1 gene plays an important role in epithelial–mesenchymal transformation (EMT), and supports miR-30b-3p as a potential biological indicator for ovarian cancer in the future.

【 授权许可】

Unknown