Es-ATG7 is required for spermatogenesis of Eriocheir sinensis and modulates p53-dependent apoptosis in germ cells

Biocell

article

SHUANG-LI HAO¹  FEI-DA NI¹  BANG-HONG WEI¹  ZHEN-FANG LI¹  TONG YANG¹  WAN-XI YANG¹ 

[1] The Sperm Laboratory, College of Life Sciences, Zhejiang University

关键词: Es-ATG7;    Spermatogenesis;    Es-p53;    Apoptosis;    Eriocheir sinensis;   

DOI  :  10.32604/biocell.2021.015178

学科分类：仪器

来源: Biocell

【 摘 要 】

Spermatogenesis is a complicated and highly regulated male gamete differentiation process that begins with the proliferation and differentiation of spermatogonia to the release of the mature spermatozoa. The autophagy-related gene atg7 has been reported as closely related to spermatogenesis and communication of Sertoli cell-germ cells in mice, including acrosome biogenesis, sperm flagellum development, and ectoplasmic specialization assembly. However, the function of es-ATG7 and its molecular regulatory mechanism during spermatogenesis in Crustacea remain largely unknown. Here, we cloned and identified es-atg7 from the testes of the Chinese mitten crabs Eriocheir sinensis and found that the expression of es-atg7 was relatively high in testes through semi-quantitative RT-PCR. The dynamic localization of es-ATG7 detected by immunofluorescence may convey information about its role in the spermatogenesis of E. sinensis . Furthermore, a knockdown of es-atg7 revealed that the malformed sperm with irregular sperm shape or loose nuclear cup and germ cell apoptosis were increased significantly. Accompanying this, we found an up-regulated expression of es-p53 during spermatogenesis in es-atg7 knockdown groups. Altogether, our results indicate that es-ATG7 plays an essential role during spermatogenesis of E. sinensis , and we demonstrated that es-ATG7 acts as an antagonist for p53-dependent apoptosis induction in this process.

【 授权许可】

CC BY