p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B

Biocell

article

Heya QIAN¹  Yan YAN²  Zhengjie SHEN¹  Lixian XU¹  Yun ZUO¹  Tao ZHU³  Yanan CHEN¹ 

[1] Department of Oncology, the Affiliated Zhangjiagang Hospital of Soochow University;Department of Pharmacology, the Affiliated Zhangjiagang Hospital of Soochow University;Department of Laboratory, the Affiliated Zhangjiagang Hospital of Soochow University

关键词: PLCε;    IP3;    Ca 2+;    Autophagy;   

DOI  :  10.32604/biocell.2019.07992

学科分类：仪器

来源: Biocell

【 摘 要 】

The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca 2+ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA levels of PLCε. The knockdown of p53, Rap2B, and PLCε significantly decreased the levels of intracellular IP3 and Ca 2+ and promoted autophagy of U2OS cells. Our results demonstrated that p53-Rap2B-PLCε-IP3 signaling pathway regulated autophagy of U2OS cells.

【 授权许可】

CC BY