A Meat-Derived Lactic Acid Bacteria, Lactobacillus plantarum IIA, Expresses a Functional Parvulin-Like Protein with Unique Structural Property

Online journal of biological sciences

article

Cahyo Budiman¹  Irma Isnafia Arief¹  Fernandes Opook²  Muhammad Yusuf¹ 

[1] IPB University;Universiti Malaysia Sabah

关键词: Peptidyl Prolyl cis-trans Isomerase;    Lactobacillus plantarum;    Parvulin;    Active Site;    Structural Homology Modelling;   

DOI  :  10.3844/ojbsci.2021.120.135

学科分类：生物科学（综合）

来源: Science Publications

【 摘 要 】

The genome sequence of a Lactic Acid Bacterium (LAB) Lactobacillus plantarum IIA contains a single gene encoding a parvulin-like protein (Par-LpIIA). This protein belongs to Peptidyl Prolyl cis-trans Isomerase (PPIase) family proteins that catalyze a slow cis-trans isomerization of cis prolyl bond during protein folding. This study aims to provide molecular and biochemical evidences of the existence of Par-LpIIA in L. plantarum IIA and have an insight into its structural properties. The result showed that the gene encoding Par-LpIIA was successfully amplified using specific primers yielding a ~900 bp amplicon indicating that the gene indeed exists in its genomic DNA. BLAST analysis confirmed that the protein is a rotamase of parvulin-like protein. Further biochemical analysis demonstrated that cell lysate of L. plantarum IIA-1A5 exhibited remarkable PPIase activity towards peptide substrate and ability to accelerate the refolding of RNase T1, with the catalytic efficiency ( k cat /KM) of 1.9 and 0.02 µM -1 s -1 , respectively. A specific inhibitor clearly inhibited the PPIase activity for parvulin-like protein with IC 50 of 230 nM confirming that the protein encoded by Par-LpIIA gene is a parvulin-like protein and expressed in an active form. Further, the three-dimensional model of Par-LpIIA showed that this protein consists of two domains of a homolog WW domain and PPIase domain with a unique active site configuration compared to human Pin1. Altogether, we then proposed the possible roles of this protein for L. plantarum IIA.

【 授权许可】

CC BY