Identification and characterization of a 14 kDa human protein as a novel parvulin‐like peptidyl prolyl cis/trans isomerase

FEBS Letters

Rahfeld, Jens-U²  Fujimori, Fumihiro¹  Uchida, Takafumi³  Tradler, Thomas²  Fischer, Gunter² 

[1] Cellular Physiology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Ibaraki 305-0074, Japan;Max-Planck Research Unit, Enzymology of Protein Folding, Weinbergweg 22, D-06120 Halle/Saale, Germany;Laboratory for Molecular Glycobiology, The Institute of Physical and Chemical Research (RIKEN), Wako Saitama 351-0198, Japan

关键词: Peptidyl-prolyl cis/trans isomerase;    Human;    Parvulin;    Pin1;    Gene;    Sequence;    DTT;    dithiothreitol;    HPLC;    high pressure liquid chromatography;    IPTG;    isopropyl β-d-thiogalactopyranoside;    LB;    Luria broth;    NH-Np;    4-nitroanilide;    PCR;    polymerase chain reaction;    PPIase;    peptidyl-prolyl cis/trans isomerase;    CsA;    cyclosporin A;   

DOI  :  10.1016/S0014-5793(99)00239-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A second member of the parvulin family of peptidyl-prolyl cis/trans isomerases was identified in a human lung cDNA library. The gene encoded a protein named hPar14 that has 131 amino acid residues and a molecular mass of 13 676 Da. Sequence comparison showed 34.5% identity to E. coli Par10 and 34% identity to human Pin1 (hPar18) within a C-terminal region of 87 or 120 amino acid residues, respectively. In comparison to the E. coli Par10, hPar14 possesses a N-terminal extension of 41 amino acid residues. This extension does not contain a polyproline II helix-binding motif typical of the known eukaryotic parvulins. The hPar14 does not accelerate the cis to trans interconversion of oligopeptides with side chain-phosphorylated Ser(Thr)-Pro moieties as hPin1 did. In contrast, it showed preference of an arginine residue adjacent N-terminal to proline. Northern blot analysis revealed expression of the gene within various human tissues like heart, placenta, liver, kidney and pancreas.

【 授权许可】

Unknown