Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells

Molecular & Cellular Toxicology

article

Adriana-Berenice, Peralta-Vega¹  Alberto, Parra-Barrera¹  del Pilar, Ramos-Godínez María²  Rebeca, López-Marure³  José, Arellano-Galindo⁴  Gutiérrez-Iglesias, Gisela¹ 

[1] Lab. de Medicina Regenerativa y Estudios en Cáncer, Departamento de Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional;Instituto Nacional de Cancerología SSA;Instituto Nacional de Cardiología “Ignacio Chávez” SSA;Hospital Infantil de México “Federico Gómez”

关键词: Mesenchymal stem cell;    Titanium dioxide;    Nanoparticle;    Toxicity;    Viability;    Cell differentiation;    Adipoblasts;    Osteoblasts;   

DOI  :  10.1007/s13273-020-00084-8

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Background Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale. Objective To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs). Methods The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated. Results TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%). Conclusion TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.

【 授权许可】

Unknown