The protective effects of echinochrome A structural analogs against oxidative stress and doxorubicin in AC16 cardiomyocytes

Molecular & Cellular Toxicology

article

Yoon, Chang Shin¹  Han, Jin¹  Kim, Hyoung Kyu¹  Mishchenko, Natalia P.²  Vasileva, Elena A.²  Fedoreyev, Sergey A.²  Shestak, Olga P.²  Balaneva, Nadezhda N.²  Novikov, Vyacheslav L.²  Stonik, Valentin A.² 

[1] National Research Laboratory for Mitochondrial Signaling, Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center (CMDC), Inje University;G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science

关键词: Echinochrome A;    Spinochromes;    Structural analogs;    Cardioprotective effect;    Doxorubicin;   

DOI  :  10.1007/s13273-019-0044-6

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Backgrounds Echinochrome A (6-ethyl-2,3,5,7,8-pen-tahydroxy-1,4-naphthoquinone) is a common naphthoquinone pigment found in the shells, spines, and coelomic fluid of sea urchins. We previously reported that echinochrome A has a cardioprotective function as an antioxidant against reactive oxygen species (ROS) induced by tert-Butyl hydroperoxide and doxorubicin. Methods In the current study, we evaluated the antioxidant activity, ATP production, and oxygen consumption rate (OCR) of seven echinochrome structural analogs (spinochromes) in AC16 human cardiomyocyte cells. The compounds included in the study had various substituents including hydroxyl (Sp B and Sp E), amino (Echm A), methoxyl (TriMeEch A), pentyl (No. 284), and hydroxypentyl (No. 285). We also investigated the effects of one dimeric spinochrome (Binaphthoquinone). Results Spinochromes exhibited enhanced antioxidant activity and ATP production. Interestingly, the hydroxylated compounds significantly enhanced the OCR and had a cardiomyocyte protective effect in the presence of doxorubicin. Conclusion Our findings indicate that echinochrome A structural analogs may have therapeutic potential for cardio-protection.

【 授权许可】

Unknown