T - Box20 inhibits osteogenic differentiation in adipose-derived human mesenchymal stem cells: the role of T - Box20 on osteogenesis

Journal of Biological Research

article

Mollazadeh, Samaneh¹  Fazly Bazzaz, Bibi Sedigheh²  Neshati, Vajiheh²  de Vries, Antoine A. F.⁴  Naderi-Meshkin, Hojjat⁵  Mojarad, Majid⁶  Neshati, Zeinab⁸  Kerachian, Mohammad Amin⁶ 

[1] Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences;Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences;Department of Food and Drug Control, School of Pharmacy, Mashhad University of Medical Sciences;Department of Cardiology, Leiden University Medical Center;Stem Cell and Regenerative Medicine Research Group, Academic Center for Education, Culture Research (ACECR);Medical Genetics Research Center, Mashhad University of Medical Sciences;Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences;Department of Biology, Faculty of Science, Ferdowsi University of Mashhad

关键词: Adipose tissue-derived mesenchymal stem cells;    Lentiviral vector;    Osteogenesis;    Transcription factor;    Tbx20;   

DOI  :  10.1186/s40709-019-0099-5

学科分类：社会科学、人文和艺术（综合）

来源: Springer

【 摘 要 】

Skeletal development and its cellular function are regulated by various transcription factors. The T-box (Tbx) family of transcription factors have critical roles in cellular differentiation as well as heart and limbs organogenesis. These factors possess activator and/or repressor domains to modify the expression of target genes. Despite the obvious effects of Tbx20 on heart development, its impact on bone development is still unknown. To investigate the consequence by forced Tbx20 expression in the osteogenic differentiation of human mesenchymal stem cells derived from adipose tissue (Ad-MSCs), these cells were transduced with a bicistronic lentiviral vector encoding Tbx20 and an enhanced green fluorescent protein. Tbx20 gene delivery system suppressed the osteogenic differentiation of Ad-MSCs, as indicated by reduction in alkaline phosphatase activity and Alizarin Red S staining. Consistently, reverse transcription-polymerase chain reaction analyses showed that Tbx20 gain-of-function reduced the expression levels of osteoblast marker genes in osteo-inductive Ad-MSCs cultures. Accordingly, Tbx20 negatively affected osteogenesis through modulating expression of key factors involved in this process. The present study suggests that Tbx20 could inhibit osteogenic differentiation in adipose-derived human mesenchymal stem cells.

【 授权许可】

CC BY-NC