Application of antibody phage display to identify potential antigenic neural precursor cell proteins

Journal of Biological Research

article

Paspaltsis, Ioannis¹  Kesidou, Evangelia²  Touloumi, Olga²  Lagoudaki, Roza³  Boziki, Marina²  Samiotaki, Martina⁴  Dafou, Dimitra⁵  Sklaviadis, Theodoros¹  Grigoriadis, Nikolaos² 

[1] Neurodegenerative Diseases Research Group, Department of Pharmacy, School of Health Sciences, Aristotle University of Thessaloniki;B’ Department of Neurology, AHEPA General University Hospital of Thessaloniki, Aristotle University of Thessaloniki;Laboratory of Physiology, Medical School, Aristotle University of Thessaloniki;Biomedical Sciences Research Center‘Alexander Fleming’;Department of Genetics, Development, School of Biology, Aristotle University of Thessaloniki

关键词: Neural precursor cells;    NPCs;    Recombinant Fab fragments;    Antibody phage display;   

DOI  :  10.1186/s40709-020-00123-4

学科分类：社会科学、人文和艺术（综合）

来源: Springer

【 摘 要 】

The discovery of neural precursor cells (NPCs) and the concomitant intensive research in the field offer regenerative medicine novel approaches, enabling it to tackle conditions, such as neurodegenerative diseases. Transplantation of NPCs is nowadays considered a cutting-edge treatment for these conditions and many related clinical trials have been already completed or are still ongoing. However, little is known about the antigenicity of NPCs, with most studies addressing the question whether their antigenicity could lead to rejection of the transplanted cells. In this study we investigated the antigenic potential of syngeneic NPCs emulsion, upon subcutaneous (s.c.) administration to wild type C57BL/6 mice, following a standard immunization protocol. The whole IgG repertoire expressed upon immunization was cloned into a Fab phage display vector. From the created phage display library, Fab expressing clones interacting with NPCs lysate proteins were selected with the biopanning technique. The IgG Fab fragment from clone 65 proved to be reactive against antigens originating from NPCs lysates and/or whole brain lysate in diverse immunological assays. Using a standard immunization protocol to administer NPCs antigens, and applying the Fab fragment phage display technique, we were able to isolate at least a monoclonal IgG Fab fragment, which interacts with different mouse brain proteins. It is not clear whether such antibodies are produced in the host organisms, following NPCs transplantation.

【 授权许可】

CC BY-NC