Neutralizing antibody against osteopontin attenuates non-alcoholic steatohepatitis in mice

Journal of Cell Communication and Signaling

article

Honda, Machiko¹  Kimura, Chiemi²  Uede, Toshimitsu²  Kon, Shigeyuki¹ 

[1] Department of Molecular Immunology, Faculty of Pharmaceutical Sciences, Fukuyama University;Division of Molecular Immunology, Institute for Genetic Medicine, Hokkaido University

关键词: Osteopontin;    Antibody;    Non-alcoholic steatohepatitis in mice;    Inflammation;    Fibrosis;   

DOI  :  10.1007/s12079-020-00554-7

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

Previously, we reported that an extracellular matrix protein, osteopontin (OPN), is involved in various autoimmune diseases using a neutralizing polyclonal antibody against OPN generated in rabbits. However, the antibody cannot be used for long-term mouse models of chronic inflammatory disease because of the induction of antibodies against anti-OPN rabbit IgG. In this study, we generated a new antibody, anti-mouse OPN mouse IgG (35B6). 35B6 inhibited the cell adhesion of mouse and human OPN to Chinese Hamster Ovary (CHO) cells or CHO cells expressing α4 or α9 integrin. It was reported that OPN is highly expressed and has an important role in a chronic liver disease, non-alcoholic steatohepatitis (NASH). 35B6 injection twice a week for 8 weeks attenuated liver inflammation and fibrosis in a NASH mouse model, suggesting 35B6 is beneficial for the treatment of NASH. 35B6 was preferable to the rabbit anti-OPN antibody for investigating the in vivo function of OPN in mouse models of long-term disease.

【 授权许可】

CC BY