Protein PEGylation for cancer therapy: bench to bedside

Journal of Cell Communication and Signaling

article

Gupta, Vijayalaxmi¹  Banerjee, Sushanta K.¹  Bhavanasi, Sneha¹  Quadir, Mohiuddin⁴  Singh, Kevin¹  Ghosh, Gaurav¹  Vasamreddy, Kritin¹  Ghosh, Arnab¹  Siahaan, Teruna J.⁵  Banerjee, Snigdha¹ 

[1] Cancer Research Unit, VA Medical Center;Department of Obstetrics and Gynecology, University of Kansas Medical Center;Department of Pathology and Laboratory Medicine, University of Kansas Medical Center;Department of Coatings and Polymeric Materials, North Dakota State University;School of Pharmacy-Pharmaceutical Chemistry, The University of Kansas

关键词: PEGylation;    Cancer;    Polyethylene glycol;    Nanoparticles;    Immunogenicity;   

DOI  :  10.1007/s12079-018-0492-0

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

PEGylation is a biochemical modification process of bioactive molecules with polyethylene glycol (PEG), which lends several desirable properties to proteins/peptides, antibodies, and vesicles considered to be used for therapy or genetic modification of cells. However, PEGylation of proteins is a complex process and can be carried out using more than one strategy that depends on the nature of the protein and the desired application. Proteins of interest are covalently conjugated or non-covalently complexed with inert PEG strings. Purification of PEGylated protein is another critical step, which is mainly carried out based on electrostatic interactions or molecular sizes using chromatography. Several PEGylated drugs are being used for diseases like anemia, kidney disease, multiple sclerosis, hemophilia and cancers. With the advancement and increased specificity of the PEGylation process, the world of drug therapy, and specifically cancer therapy could benefit by utilizing this technique to create more stable and non-immunogenic therapies. In this article we describe the structure and functions of PEGylation and how this chemistry helps in drug discovery. Moreover, special emphasis has been given to CCN-family proteins that can be targeted or used as therapy to prevent or block cancer progression through PEGylation technology.

【 授权许可】

CC BY