Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer

Pathology oncology research: POR

article

Rivera-Franco, Monica M.¹  Leon-Rodriguez, Eucario²  Torres-Ruiz, José J.³  Gómez-Martín, Diana⁴  Angles-Cano, Eduardo⁵  de la Luz Sevilla-González, María¹ 

[1] Section of Graduate and Research Studies, Instituto Politecnico Nacional. Plan de San Luis y Diaz Miron s/n;Department of Hematology and Oncology;Department of Hematology and Oncology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran;Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud;Inserm UMR_S1140 Innovative Therapies in Haemostasis, Faculté de Pharmacie de Paris

关键词: Neutrophil extracellular traps;    Breast Cancer;    Metastasis;   

DOI  :  10.1007/s12253-019-00763-5

来源: Springer

【 摘 要 】

Recently, neutrophil extracellular traps (NETs), three-dimensional structures formed of neutrophil enzymes such as neutrophil elastase (NE) and nuclear components (DNA), have been associated with progression in different types of cancer. However, data remain scarce in breast cancer. Thus, the aim of this study was to associate NETs with clinical stages of breast cancer. A prospective analysis was performed in 45 plasma samples of female patients with newly diagnosed breast cancer. NE-DNA complexes were evaluated by ELISA. Optical density was dichotomized at the median for comparisons (low and high levels of NE-DNA). The most frequent clinical stage was localized (n = 28, 62%) followed by regional (n = 13, 29%) and distant (n = 4, 9%). Higher levels of NE-DNA complexes were observed in regional and distant stages compared to localized disease (68% vs 32%, p = 0.034). No differences were observed when comparing other clinical characteristics between both groups. We demonstrated that the levels of NETs increase in proportion to the stage of the disease, observing higher levels of NE-DNA complexes in regional and metastatic disease, which coincides with the proposed mechanism by which cancer progression and metastasis might result from the formation of NETs.

【 授权许可】

Unknown