The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci

eLife

Paul Turner¹  William P Hanage²  Robert F Breiman³  Kwan Soo Ko⁴  Jae-Hoon Song⁴  Mark PG van der Linden⁵  Herminia de Lencastre⁶  Raquel Sá-Leão⁷  Nicholas J Croucher⁸  Joshua C D'Aeth⁸  Stephanie W Lo⁹  Rebecca A Gladstone⁹  Stephen D Bentley⁹  Lesley McGee^1,10  Bernard Beall^1,10 

[1] Cambodia Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia;Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom;Center for Communicable Disease Dynamics, Harvard T.H. Chan School of Public Health, Boston, United States;Department of Global Health, Rollins School of Public Health, Emory University, Atlanta, United States;Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea;Institute for Medical Microbiology, National Reference Center for Streptococci, University Hospital RWTH Aachen, Aachen, Germany;Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal;Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, United States;Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal;MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom;Parasites & Microbes, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom;Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, United States;

关键词: streptococcus pneumoniae;    AMR;    recombination;    genomics;    epidemiology;    Other;   

DOI  :  10.7554/eLife.67113

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1-type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking homologous arms. Phylodynamic analysis of the most commonly sampled Tn1207.1-type insertion in PMEN9, originating from a commensal and disrupting a competence gene, suggested its expansion across Germany was driven by a high ratio of macrolide-to-β-lactam consumption. Hence, selection from antibiotic consumption was sufficient for these atypically large recombinations to overcome species boundaries across the pneumococcal chromosome.

【 授权许可】

CC BY