Aedes species (Diptera: Culicidae) ecological and host feeding patterns in the north-eastern parts of South Africa, 2014–2018

Parasites & Vectors

B. D. Brooke¹  L. E. O. Braack²  M. Schrama³  E. E. Gorsich⁴  Marietjie Venter⁵  M. M. Guarido⁵  T. Johnson⁶  M. A. Riddin⁷  A. P. G. Almeida⁸ 

[1] Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases/NHLS, Johannesburg, South Africa;Wits Research Institute for Malaria, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa;Faculty of Health Sciences, UP Institute for Sustainable Malaria Control, University of Pretoria, Pretoria, South Africa;Malaria Consortium, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Institute of Environmental Sciences, Leiden University, Leiden, The Netherlands;School of Life Sciences, University of Warwick, Coventry, UK;The Zeeman Institute for Systems Biology and Infectious Disease Epidemiology Research, University of Warwick, Coventry, UK;Zoonotic Arbo- and Respiratory Virus Program, Department Medical Virology, Faculty of Health Sciences, Centre for Viral Zoonoses, University of Pretoria, Pathology Building, Prinshof Campus South, Private Bag X323, Gezina, 0031, Pretoria, South Africa;Zoonotic Arbo- and Respiratory Virus Program, Department Medical Virology, Faculty of Health Sciences, Centre for Viral Zoonoses, University of Pretoria, Pathology Building, Prinshof Campus South, Private Bag X323, Gezina, 0031, Pretoria, South Africa;Department of Biological Sciences, Copperbelt University, Kitwe, Zambia;Zoonotic Arbo- and Respiratory Virus Program, Department Medical Virology, Faculty of Health Sciences, Centre for Viral Zoonoses, University of Pretoria, Pathology Building, Prinshof Campus South, Private Bag X323, Gezina, 0031, Pretoria, South Africa;Faculty of Health Sciences, UP Institute for Sustainable Malaria Control, University of Pretoria, Pretoria, South Africa;Zoonotic Arbo- and Respiratory Virus Program, Department Medical Virology, Faculty of Health Sciences, Centre for Viral Zoonoses, University of Pretoria, Pathology Building, Prinshof Campus South, Private Bag X323, Gezina, 0031, Pretoria, South Africa;Institute of Tropical Medicine and Hygiene (IHMTNOVA), Medical Parasitology Unit/GHTM, NOVA University of Lisbon, Lisbon, Portugal;

关键词: Aedes;    Mosquitoes;    Disease vectors;    Africa;    South Africa;   

DOI  :  10.1186/s13071-021-04845-9

来源: Springer

【 摘 要 】

BackgroundThere is a paucity of recent data and knowledge on mosquito diversity and potential vectors of arboviruses in South Africa, with most of the available data dating back to the 1950s–1970s. Aedes and Culex species are the major vectors of some of the principal arboviruses which have emerged and re-emerged in the past few decades.MethodsIn this study we used entomological surveillance in selected areas in the north-eastern parts of South Africa from 2014 to 2018 to assess mosquito diversity, with special emphasis on the Aedes species. The impact of trap types and environmental conditions was also investigated. Identification of the blood meal sources of engorged females collected during the study period was carried out, and DNA barcodes were generated for selected species.ResultsOverall, 18.5% of the total Culicidae mosquitoes collected belonged to the genus Aedes, with 14 species recognised or suspected vectors of arboviruses. Species belonging to the Neomelaniconion subgenus were commonly collected in the Bushveld savanna at conservation areas, especially Aedes mcintoshi and Aedes circumluteolus. Aedes aegypti was present in all sites, albeit in low numbers. Temperature was a limiting factor for the Aedes population, and they were almost exclusively collected at temperatures between 18 °C and 27 °C. The cytochrome oxidase subunit I (COI) barcode fragment was amplified for 21 Aedes species, and for nine of these species it was the first sequence information uploaded on GenBank.ConclusionThis study provides a better understanding of the diversity and relative abundance of Aedes species in the north-east of South Africa. The information provided here will contribute to future arboviral research and implementation of efficient vector control and prevention strategies.Graphical abstract

【 授权许可】

CC BY