Screening of some weed extracts for ovicidal and larvicidal activities against dengue vector Aedes aegypti

The Journal of Basic and Applied Zoology

Sarika Torawane¹  Digambar Mokat¹  Samadhan Phuge²  Radhakrishna Pandit²  Ramnath Andhale² 

[1] Department of Botany, Savitribai Phule Pune University, 411007, Pune, India;Department of Zoology, Savitribai Phule Pune University, 411007, Pune, India;

关键词: Aedes aegypti;    Gut histology;    Larvicidal activity;    Ovicidal activity;    Plant extract;   

DOI  :  10.1186/s41936-021-00233-y

来源: Springer

【 摘 要 】

BackgroundThe use of plant-based formulations is an easy and economical method to control insect pests. The objectives of the present study were to evaluate ovicidal and larvicidal potential of ethanolic leaf extracts of four plants, Cyathocline purpurea, Blumea lacera, Neanotis lancifolia, and Neanotis montholonii, and their effects on gut histology of Aedes aegypti (Diptera: Culicidae) larvae. We identified the phytochemicals present in the ethanolic extracts of these plants by LC-MS analysis. The eggs and larvae of A. aegypti were exposed to four concentrations (0.05, 0.1, 0.2, and 0.3 mg/mL) of crude ethanol extracts for different time durations. We observed egg hatching for 48 h, mortality, and gut histology of the 3rd instar larvae after 24, 48, and 72 h of treatments.ResultsLC-MS analysis revealed the presence of various active compounds such as alkaloids, tannins, saponins, and flavonoids in the ethanol extracts of all these plants. The extracts of all plants showed ovicidal activity. The extracts of C. purpurea showed the highest larvicidal activity (LC50 at 72 h = 0.088 mg/mL) followed by B. lacera (LC50 at 72 h = 0.758 mg/mL) while N. lancifolia and N. montholonii extracts did not show significant larvicidal activity. All plant extracts affected gut morphology in concentration and treatment duration-dependent manner. The plant extracts used in the present study have ovicidal properties and affected the gut histology of A. aegypti larvae.ConclusionThese results suggest that C. purpurea has the highest larvicidal activity against A. aegypti larvae and can be further evaluated for application purposes.

【 授权许可】

CC BY