Anti-Mayaro virus activity of Cassia australis extracts (Fabaceae, Leguminosae)

Particle and Fibre Toxicology

Ricardo M Kuster⁴  Wanderson Romão³  Davis Ferreira⁶  Alice Sato¹  Marcelo D F de Meneses⁶  Tiago S Salles⁵  Naomi K Simas²  Kassia C W Spindola⁴ 

[1] Botanical Department. Center of Biological and Health Sciences, Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil;Natural Product and Food Department. Center of Health Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Petroleomic and Forensic Laboratory, Department of Chemistry, Federal University of Espírito Santo, Vitória, 29075-910, ES, Brazil;Natural Product Research Institute, Center of Health Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Chemistry Institute, Biochemistry Department, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Microbiology Institute, Virology Department, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

关键词: Aedes aegypti;    Larvicidal activity;    MAYV;    Antiviral;    Tannins;    Flavonoids;    Cassia australis;   

Others  :  1148976
DOI  :  10.1186/s13071-014-0537-z

 received in 2014-07-23, accepted in 2014-11-12,  发布年份 2014

【 摘 要 】

Background

The arthropod-borne Mayaro virus (MAYV) causes ‘Mayaro fever’, a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Studies showed that the virus could also be transmitted by the mosquito Aedes aegypti. Recently, MAYV has attracted attention due to its likely urbanization. To date, there are no drugs that can treat this illness.

Methods

Fractions and compounds were obtained by chromatography from leaf extracts of C. australis and chemically identified as flavonoids and condensed tannins using spectroscopic and spectrometric techniques (UV, NMR, and ESI-FT-ICR MS). Cytotoxicity of EtOAc, n-BuOH and EtOAc-Pp fractions were measured by the dye-uptake assay while their antiviral activity was evaluated by a virus yield inhibition assay. Larvicidal activity was measured by the procedures recommended by the WHO expert committee for determining acute toxicity.

Results

The following group of substances was identified from EtOAc, n-BuOH and EtOAc-Pp fractions: flavones, flavonols, and their glycosides and condensed tannins. EtOAc and n-BuOH fractions inhibited MAYV production, respectively, by more than 70% and 85% at 25 μg/mL. EtOAc-Pp fraction inhibited MAYV production by more than 90% at 10 μg/mL, displaying a stronger antiviral effect than the licensed antiviral ribavirin. This fraction had an excellent antiviral effect (IC₉₀ = 4.7 ± 0.3 μg/mL), while EtOAc and n-BuOH fractions were less active (IC₉₀ = 89.1 ± 4.4 μg/mL and IC₉₀ = 40.9 ± 5.7 μg/mL, respectively).

Conclusions

C. australis can be used as a source of compounds with anti-Mayaro virus activity. This is the first report on the biological activity of C. australis.

【 授权许可】

   
2014 Spindola et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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