Targeted imaging of esophageal adenocarcinoma with a near-infrared fluorescent peptide

BMC Gastroenterology

Meng Li¹  Rui Zhang²  Lei liu³  Yanglin Pan⁴  Siming Ning⁴  Hao Hu⁴  Shaopeng Liu⁴  Xiaoyu Kang⁴  Kaichun Wu⁴  Zuhong Tian⁴  Xuegang Guo⁴ 

[1] Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China;Department of Critical Care Medicine, Shaanxi Provincial Cancer Hospital, Xi’an, Shaanxi, People’s Republic of China;College of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China;Department of Gastroenterology, Tangdu Hospital of the Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China;State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 127 Changle West Road, 710032, Xi’an, Shaanxi, People’s Republic of China;

关键词: Esophageal carcinoma;    Peptide;    Near infrared;    Probe;   

DOI  :  10.1186/s12876-021-01840-3

来源: Springer

【 摘 要 】

BackgroundTargeted optical imaging offers a noninvasive and accurate method for the early detection of gastrointestinal tumors, especially for flat appearances. In our previous study, a sequence of SNFYMPL (SNF) was identified as a specific peptide to bind to esophageal carcinoma using phage-display technology. This study aimed to evaluate the tumor-targeting efficacy of Cy5.5-conjugated SNF probe for imaging of esophageal carcinoma in vitro and in vivo.MethodsThe SNF-Cy5.5 probe was synthesized and then identified using High Performance Liquid Chromatography (HPLC) and mass spectrometry (MS). Confocal fluorescence imaging and Flow cytometry analysis were performed to evaluate the binding specificity and the receptor binding affinity of SNF-Cy5.5 to OE33. In vivo imaging was performed to evaluate the targeting ability of SNF-Cy5.5 to esophageal carcinoma.ResultsThe confocal imaging and flow cytometry analysis showed that SNF-Cy5.5 bound specifically to the plasma membrane of OE33 cells with a high affinity. In vivo, for non-block group, SNF-Cy5.5 probe exhibited rapid OE33 tumor targeting during 24 h p.i. and excellent tumor-to-background contrast at 2 h p.i. For the block group, SNF-Cy5.5 was not observed in the mice after 4 h p.i. Ex vivo imaging also revealed that a higher fluorescent signal intensity value of the tumors was clearly observed in the non-block group than that in the block group (2.6 ± 0.32 × 109 vs. 0.8 ± 0.08 × 109, p < 0.05).ConclusionsSNF-Cy5.5 was synthesized and characterized with a high efficiency and purity. The higher affinity, specificity, and tumor targeting efficacy of SNF-Cy5.5 were confirmed by in vitro and in vivo tests. SNF-Cy5.5 is a promising optical probe for the imaging of esophageal adenocarcinoma.

【 授权许可】

CC BY